Sentence examples for spin concentrated from inspiring English sources
Subsequently, the enzymes were dialysed overnight into 25 mM Tris HCl, 150 mM NaCl, pH 7.5 and then spin concentrated.
The enzymes were then cleaved overnight at 4°C with PreScission protease and the supernatants containing the proteins were then spin concentrated.
This was spin concentrated to 5 ml.
Purified XcOGT protein was spin concentrated to 10 mg/ml.
The spin density (SD) is a measure of free spin concentrated on the benzylic carbon after hydrogen atom abstraction (Beasley et al., 2009).
The spinning concentrates the platelets in the plasma, the liquid part of blood.
Its effort focused on developing centrifuges, a standard method in which fast spinning concentrates U-235, the uranium isotope used in making bombs.
After overnight dialysis against 100 mM sodium phosphate buffer, pH7.0, the samples were concentrated using Amicon Ultra-15 100,000 MWCO spin concentrators as described by the manufacturer.
However, the collected multimers can be concentrated in spin concentrators without causing any dissociation into dimers (data not shown).
The elution fractions were pooled and concentrated in Millipore Spin concentrators (< 3 kDa pore size), heated with 6×SDS/PAGE sample buffer containing 2-mercaptoethanol for 10 min at 95°C and analysed by SDS/PAGE using Mini-PROTEAN any kD TGX Precast Gels (Bio-Rad Laboratories GmbH).
The 8.9 kDa protein was purified using QhyperD fractionation (Biosepra Inc., Malborough, MA, USA) and concentrated on YM50 spin concentrators (Millipore, Billerica, MA, USA).
