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The similarly derived YFP spillover factor (YSF) was 0.09±0.04 (n=46).
Spillover factor measurements were repeated throughout the course of our studies and did not change significantly.
The CFP spillover factor (CSF) is the intensity of the Smc1-CFP signal in the FRET channel, divided by its intensity in the CFP channel, and was found to be 0.34±0.04 (n=48).
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CFP and YFP spillover factors were measured in strains Y1967 and Y1970, expressing Smc1-CFP and Smc1-YFP, respectively.
Therefore, spillover factors are first determined, and FRETR is measured as the ratio of the observed FRET intensity over the expected spillover (see Materials and methods, and Muller et al, 2005, for details).
This species difference is probably related to anatomical differences including a substantially lower number of airway branching in the mouse compared to the human lung that may result in a faster spillover of damaging factors from the conducting airways to the distal airspaces in mice.
Results suggest that the practice of allowing swine to roam freely is a significant risk factor for spillover of influenza from humans into swine populations.
The practice of allowing swine to roam free, often combined with poor hygienic practices, may be an important factor in spillover of pH1N1 from humans to swine in northern Cameroon.
Underlying factors that might explain these fluctuations are contextual determinants (mainly environmental factors) and spillover effects into the human population, which trigger epidemics.
Between December 2009 and August 2012, we conducted research at 12 sites in Cameroon to assess prevalence rates and risk factors for spillover.
Default risk includes a systematic component from a conceptual perspective, aside from company-specific idiosyncratic risk, which is contingent on macroeconomic factors and causes spillover effects as well as industry-specific yet time-varying risk factors (Giesecke and Kim 2011; Vassalou and Xing 2004).
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