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Spike experiments at 37 °C were paused after 20 min. The spike time for each temperature was set to the start of constant protein production determined by preliminary experiments with our Att-1-SFGFP construct.
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First, spike trains were obtained by binning the spike times for each neuron with 1 ms resolution.
Briefly, the simulated voltage traces were generated by drawing spike times for a given firing rate, assigning an iP or a CW to each spike time, adding white Gaussian noise, and filtering the resulting signal similarly to the experimental apparatus.
Tick marks indicate spike times for 10 repetitions of the same light stimulus.
To determine the time course of the NPC, we first aligned the spike times for each trial to either the target onset or the saccade onset.
Before modeling the input spikes for any stimulus presentations, a template spike train is generated separately for each presynaptic neuron using equation 4. The actual input spike times for a single stimulus presentation are generated by jittering the spike times in each template with Gaussian noise of mean 0 and variance σ.
Therefore, in accordance with previous findings (Zhang et al., 2009; Yang and Dani, 2014), the presence of DA during the coordinated spiking activity widens the spike time interval for induction of t-LTP.
The spike raster shows the spike times of neurons for a single trial; the spikes from the same group are plotted on the same row.
(E ) Exogenous DA widens the spike time window for induction of t-LTP.
(C ) Endogenous DA widens the spike time window for induction of t-LTP.
To compute the locking of neuronal firing to whisker positions, spike time stamps for each cell were aligned to whisker traces and binned in a circular fashion before being normalized for phase occupancy.
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