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The accuracy was confirmed by the analysis of certified reference materials (Drinking Water Standard APS 1071, Fresh Water Sediment 3 RTC 016, Oyster Tissue NIST 1566b, Tomato Leaves NIST 1773a), the Spike test showed recoveries between 90 and 110%.
Through the melting curve analysis, comparisons for gene copy numbers among the three clades and spike test for field study, our qPCR assay was reliable to quantify the cell numbers of each clade.
There was strong linear correlation (R2 > 0.990) between cell abundances as estimated by qPCR assay and direct counting via light microscope in spike test, and 0.24 (clade I), 0.25 (clade II) and 0.33 (clade III) P. pungens cells per mL were detected markedly upon the use of specific two-primer set.
Result of spike test at depths of a 60 km, b 100 km, c 140 km, and d 180 km is shown.
CYN was recoverable in a spike test with tap water [ 125].
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Accuracy was evaluated by spike tests in the analyzed samples.
The accuracy was evaluated by spike tests in oil-refinery effluent samples and analysis of a vegetable certified reference material (NIST 1571, orchard leaves).
Analysis of a certified reference material (Trace elements water, NIST 1643e) and spike tests with recoveries between 84% and 123% showed that the proposed method presents adequate accuracy.
The accuracy was confirmed by cadmium with spike tests with recuperations varying from 92% to 103%, procedure was applied for cadmium determination in drinking water samples collected from Salvador City, Bahia, Brazil.
Accuracy was confirmed by copper determination in the standard reference material, NIST SRM 1570a trace element units in Spinach Leaves and by spike tests with recovery levels ranging from 93 to 100%; the procedure was applied for copper determination in tobacco leaf samples collected in Cruz das Almas City, Bahia, Brazil.
Symbols are as in Fig. 4 Fig. 10 Results of spike tests for phase velocity distributions.
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