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In this way, a known number of natural isotopes can be mixed with a known amount of spike and the concentration in the spike solution determined from the ratio of the masses.
As explained below, the designed technique facilitates also determination of butyrylcholinesterase (BChE) activity both in the buffer and in spike solution with human plasma.
The AnApureTM ICP standard of 1,002 (±10) mg/L Sr (lot no. AEP-130-726) was diluted to 21.480 μg/g solution in 2% HNO3 (the specific gravity of 2% HNO3 = 1.028 g/mL) and then was admixed with the spike solution.
The first spike solution contained myo- and scyllo-IP6, while a second spike solution contained neo- and d- chiro-IP6.
Briefly, isotopically enriched Hg spike solution was prepared and calibrated using NIST Standard Reference Material (SRM) 3133 Hg Spectrometric Solution.
The ablated aerosol of either standard or sample is mixed with the nebulized isotope-enriched spike solution or blank solution.
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After every metal spike, solutions were stirred for equilibration, and N2 was bubbled to deoxygenate.
Recently, Seok [19] considered the spike solutions of (1.1) for a more general nonlinear term.
For single boundary spike solutions with Neumann boundary condition, please see [6, 12 15]etc.
In [11], using a Lyapunov-Schmidt reduction, Floer and Weinstein established the existence of single and multiple spike solutions.
Floer and Weinstein in [11] first studied the existence of single and multiple spike solutions based onthe Lyapunov-Schmidt reductions.
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