In this step, a "cap-fitting" algorithm is used to automatically detect a spike free segment and then the mean, standard deviation and autocorrelation function of the noise are computed.
The spike gene segment had a 99.7% nucleotide percentage identity (99.4% amino acid identity) with Colorado/2013 and clustered with the non North American INDEL strains.
When PEDV was first detected in the United States, the University of Minnesota Veterinary Diagnostic Laboratory was only sequencing the PEDV spike gene segment to clarify the phylogenetic relationship between PEDV strains.
In this scenario, the "initial segment" spike is generated first, and antidromically invades the soma before the "somato-dendritic" spike is generated [9], thus leading to a double-component shape of the AP upstroke.
The second step involves generating optimal templates of the spikes from a segment containing both noise and multiunit activity.
A predicted binding site that doesn't fall within these 4 cases is labelled as a FP, while a missed spike-in DNA segment constitutes a FN.
Here, a predicted peak is identified as a TP if a predicted binding site overlaps with the true starting or ending location of a spike-in DNA segment (see Figure 2).
At the jump point, the fast directions of the flow take over, which causes the formation of a small spike as the orbit segment leaves S; see also Figure 5c.
Also, we carefully excluded every segment with spikes on the scalp, therefore we believe that the presence of post-spike slow waves did not influence our findings (Urrestarazu et al., 2006).
Thus, KCNQ2/3 channel activity may influence intrinsic excitability at the initial segment, where fast spikes as well as spike ADPs are initiated [64], [65].
This phenomenon becomes more evident when we take a look at the projections of all spike-triggered stimulus segments on both PC1 and PC2.
