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A principal reason for discrepancies with previous results may be that microscopy, electronic and computing equipment have developed considerably in the past 20 years, and thus allow for much finer delineation of spike heights than the original 1987 study [ 11], particularly among F1 hybrids.
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In a situation without bone fusion, the spike row, the spike oblique, and the spike height were especially important factors.
In a situation with bone fusion, the spike row, the spike height, and the inner diameter were the most significant factors.
Here, the optimum combination has been found to be the conical spike type, a spike height of 2 mm, a spike diameter of 2.2 mm, an oblique geometry, 11 rows per 28 mm, and an inner diameter of 20 mm.
The optimum combination has been found to be the pyramidal spike type, a spike height of 2 mm, a spike diameter of 2.2 mm, an oblique geometry, 11 rows per 28 mm, and an inner diameter of 10 mm.
These parameters included the peak, valley, spike height, spike width, peak time, valley time and two user-defined voltage values.
The gradual decline in spike height seems to reflect WST11 clearance and possibly development of vascular photo-damage (Fig. 2H).
The spike sorting capabilities of Spike 2 software were used to identify individual neurons on the basis of spike height and shape.
Spike clusters were defined by visual inspection using a convex-hull method (SpikeSort3D Neuralynx Inc., Bozeman, MT) along a five-dimensional feature vector including: 1) peak and 2) valley amplitude, 3) spike height, 4) spike waveform energy and 5) the 8th sample amplitude.
The axes of cluster space used were spike height, valley, and energy.
In addition, the spike height from threshold and the minimum membrane potential reached following the spike were measured.
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