Sentence examples for spike factor from inspiring English sources

A raw readout of 10 by the current Affymetrix GeneChip system corresponds to approximately one copy per cell in mouse liver (spike factor = 0.2), whereas in mouse thymus (spike factor = 0.01) it corresponds to approximately 0.05 copy per cell.

By referring to the amount of DNA in a diploid cell and employing a "spike factor" determined by the ratio of total RNA to genomic DNA in a tissue type (Table 1), the spike mRNAs were calculated to correspond to 468.1, 156.0, 52.0, 17.3 and 5.8 copies per cell (diploid), respectively, for the mouse liver samples (spike factor = 0.2).

As described above, using a combination of DNA content and the spike factor of the sample, the GSC spike mRNAs become direct indicators of the copy numbers (C') per cell.

For Q-PCR, the same spike factor corresponds to Ct values ranging approximately from 17 to 27, which is well within the linear range of Q-PCR (data not shown).

As a result, the GSC spikes AFFX-TrpnX-3_at, AFFX-DapX-3_at, AFFX-PheX-3_at, AFFX-LysX-3_at and AFFX-ThrX-3_at correspond approximately to 5.8, 17.3, 52.0, 156.0 and 468.1 copies per cell (per diploid DNA template) for mouse liver sample homogenates, where the spike factor = 0.2.

In practice, for the Affymetrix GeneChips, the spike factor is set so that the five GSC spikes cover the range of "Present" calls given by the Affymetrix system, which corresponds to approximately 80 to 7000 in raw readouts given by the Affymetrix MAS5.0 software.

Spike factors have been pre-determined for various organs/tissues to reflect differences in their total RNA/genomic DNA ratios (cf. Table 1).

In Equations 2, 4, the term ΔT is defined as the spike slope factor (Fourcaud-Trocmé et al., 2003) and it measures the sharpness of the spike initiation.

But the trend in the United States trade balance could be unsettled in coming months as the price spikes are factored in.

Recent studies have highlighted the duration of the complex spike as a factor that may regulate the induction of plasticity by the climbing fibers.

Quality control values, present calls, background, noise, scaling factor, spike controls, and the 3'/5' ratios of glyceraldehyde-3-phosphate dehydrogenase (AT3G04120) and actin (AT5G09810) showed minimal variation between samples.