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We assess individual probe performance by fitting the Langmuir isotherm to the hybridization intensity of spiked probes vs known spike concentration.
Recoveries were calculated by subtracting the background level in the blank serum from the level in the spiked serum and dividing by the original spike concentration.
The results cannot conclusively be attributed to a nano- effect due to the higher spike concentration of Ag in the AgNP treated sludge compared to the Ag+ sludge.
When lower spike concentration was used, extraction recoveries were generally good (>80%%), except for E3-3S and E1-3S (70.9 % for both compounds).
Accuracy was evaluated by comparing the results of spiked tap water, river water, wastewater and urine samples (50 200 ng L−1 for water samples and 500 5000 ng L−1 for urine samples) with the nominal spike concentration.
A very low spike concentration (50 or 200 ng L−1) was used to perform validation tests and since E1-3S was the compound with the weakest signal in this method (Fig. 5), it was acceptable that it presented lower precision during the analysis.
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Therefore, there are altogether 34 unique labeled and spiked 50 mers against a human cRNA background for each of the spike concentrations.
The claimed mLR concentration response range of the tests was confirmed within reasonable limits, although the false negative and false positive rates were significant for spike concentrations below 2.5μg/LL (Recreational Strip Test).
This factor was obtained using linear regression and information about the known spike concentrations per cell.
These were tested at five different bacterial spike concentrations in triplicate per run, for four runs.
Sensitivity was only decreasing when spike concentrations were very low (cf. charts 2 – 6 in figure 4) but this was also observed with dChip or with RMA and SAM.
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