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Electrophysiological response to stimulation was assessed by complex and simple spike analysis.
To avoid the issue of inducing false correlations, we have used RPM rather than RPKM except for spike analysis.
A spike analysis was carried out using the Spike 2 software program for Windows (Version 2.01).
Also, the spike analysis (threshold, amplitude) is from long depolarizing steps while the AHP analysis is from brief pulses.
Moreover, single spike analysis of combined EEG and MEG recordings is favorable to take full benefit of the complementarities between these two modalities (Pataraia et al. 2005).
We did not consider paths of length two because only 40% of them were context-sensitive according to the SPIKE analysis (Fig. 4).
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We have added shading to all relevant graphs to show the analysis intervals, with simple-spike analysis intervals in light pink and complex-spike analysis intervals in light gray.
The difference in the firing rate averaged across the simple-spike analysis interval was statistically-significant (paired t-test, p=0.002, n = 90).
With this aim, we have prototyped original software that performs an automated Ca2+ spiking analysis (CaSA) based on our recent studies where a prototypal computational model was formalized to simulate Ca2+ spiking dynamics [ 16].
As a contribution, we have developed automated Ca2+ spiking analysis (CaSA) software that performs i) automated peak detection, ii) statistical analyses based on the detected peaks, iii) autocorrelation analysis of peak-to-peak intervals to highlight major traits in the spiking pattern.
Spike-analysis was performed offline using the discharges generated by mechanical stimulation of meningeal receptive fields as templates (Spike 2 software application, Cambridge Electronic Design).
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