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Five spike-in controls (Ambion ArrayControl™ RNA Spike 3 through 7) were added to each of our 12 samples in decreasing amounts following a log2 scale.
Six-rowed spike 2 (vrs2), six-rowed spike 3 (vrspikend six-rowed spike 4 (vrs4) are independent recessive genes, which are located on chromosome 5HL, 1HL and 3HL, respectively (Fig. 2; Lundqvist et and, 1997).
In the two treatments added with the same Alien® mRNA spike-in mix (i.e., control vs. TNT), we identified 957 significant genes including 5 spots of alien spike 3 and 9 spots of negative controls (water and printing buffer), which is reasonable given the large number of significant genes called and the selected inference conditions.
Under the selected conditions (95% confidence level and 10 allowable false discoveries), we identified 151 and 96 significant genes in the RDX- and mixture-treated worms, respectively, without any alien spike 3 (Table 2 and Additional file Additional file 2: Table S2), suggesting no false positive from the spike-in mRNAs.
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Spike 80DF has been confirmed as the chemical used.
Inflorescence: spike, 5--20 mm wide, ovate to short-cylindric.
# 0: Using monkey spike, 1: Using original data to update DP cells.
Inflorescence: spike 1.5--6 cm; axis densely and finely tomentose; bract slightly < calyx, lanceolate.
In this study, we selected seven epitopes from the spike (S1), spike (S2), and nucleocapsid (N) protein and constructed a multi-epitope DNA vaccine.
Even with the spike, 2014 was still the fourth safest year for cops since 1959.
Results showed that, following the day of spike, NP2EO degraded rapidly.
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