In terms of the size effect, we want to highlight the fact that a scattering peak displaced towards the reddish wavelength for X values larger than 1 has been observed in all cases, independently of the ice (CO and CO2), the shape (sphere, spheroid with axial proportion 5, DW1999, and aggregates), or the inclusion of other constituents, such as silicate.
Spheroids were harvested using BD cell recovery solution, on ice, and broken open with 1% (v/v) trypsin to release the luminal dyes.
Massie, I., Selden, C., Hodgson, H. & Fuller, B. Cryopreservation of encapsulated liver spheroids for a bioartificial liver: reducing latent cryoinjury using an ice nucleating agent.
Cells dissociated from the spheroids were collected into stirred tubes containing complete medium on ice, while the remaining aggregates remained in the chamber.
The day after, spheroids were collected, centrifuged and the spheroid pellet was mixed with methocoel-mix The collagen gel was prepared on ice and 500 μl of collagen gel were added to the spheroid/methocoel solution, mixed by pipetting and seeded on a 24-well culture dish for 30 min in an incubator.
The results of our experiments suggest that the spheroids observed on a strut of the Phoenix lander formed on water ice splashed during landing [ Smith et al., 2009; Rennó et al., 2009].
The harvested spheroid culture cells or cancer cells from the peritoneal cavity were washed in ice-cold PBS and lysed in RIPA buffer (10 mM Tris HCl [pH7.4], 0.1% nonidet-P-40, 0.1% sodium deoxycholate, 0.1% SDS, 150 mM NaCl, 1 mM EDTA, 10μg/mL aprotinin, and phosphatase inhibitor cocktail [Nacalai Tesque, Inc., Kyoto, Japan]).
a, Schematic of spheroid generation.
Unh! Hmph! Accursed little spheroid, accursed clubbed stick!
The fate of a bouncing spheroid determines one's fortunes.
Such a figure is called a spheroid.
