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Carboxymethyl cellulose (CMC) was added at a final concentration of 0.8% to keep the fluid flow slow, and the spheres were quantified using an inverted phase contrast microscope (Olympus Co).
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Maximum perpendicular sphere diameters were quantified using a Zeiss Observer Z1 inverted microscope and Axiovision 4.8 imaging software (Carl Zeiss, Goettingen, Germany) and used to calculate sphere volumes.
Proteins were quantified using Qubit® 2.0 Fluorometer.
Immunoblots were quantified using ImageJ.
RNA samples were quantified using spectrophotometry.
Proteins were quantified using Bradford protein assay.
PCR products were quantified using ImageJ.
Alternative splicing isoforms were quantified using ImageJ.
Images were quantified using Image J software.
Blots were quantified using ImageJ software.
Band intensities were quantified using ImageJ (National Institutes of Health).
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