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The Dutch English connection in the person of William III, the continuing threat of Louis XIV, and a succession of shattering events in various spheres stimulated a vast production of cartoons from the 1680s on.
When cultured with myofibroblasts the collagen spheres stimulated a prolonged significant increase in secretion of the angiogenic growth factor, vascular endothelial growth factor (VEGF), compared with cells alone.
(G ) Human C-peptide secretion assay of 4TFM spheres stimulated by the indicated secretagogues and drugs.
DOI: http://dx.doi.org/10.7554/eLife.06104.012 Consistent with the q-PCR data, immunofluorescence labeling showed that the percentages of K5-positive cells in spheres stimulated with HGF for 9 10 days were six times higher that that of untreated cultures.
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On the surface of the reflector, piezoelectric elements arranged to form part of a sphere are stimulated with a high-energy electrical pulse.
Control polycaprolactone (PCL) spheres failed to stimulate a similar prolonged increase in VEGF secretion.
TNF significantly stimulated tumor-like sphere formation in both cell lines, indicating that the TNF effect is BRAFV600E-independent and confirming that TNF expands the melanoma SC pool.
These, in turn, are stimulated by the expansion of tiny spheres of a material called cuticula.
These will encompass novel, smart, functionalised and hybrid materials for diverse applications whose discovery and routes to bio-production will be stimulated by the fusion of new technologies positioned across physical, digital and biological spheres.
To prove that K5-expressing cells in HGF-treated spheres originated from luminal progenitors, we isolated Lu-pos cells from Blg-Cre; R26 mouse mammary glands and stimulated them with HGF for 9 10 days.
Fully stimulated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com