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A similar idea involving the use of Benthos glass spheres, which could survive the pressure, was scuppered when the cost of the number of spheres required was put at over $238 million.
During the phase of the NPC protocol where cells are plated in ultra-low adherence 6-well plates, the phESC required particularly gentle feeding techniques as the spheres required to achieve appropriate cell-cell signaling were more prone to dissociation than those of their hESC counterparts (Fig. 5a, 5b).
2 patients were excluded, 1 because of inadequate placement of the implanted tantalum spheres required for RSA and the second because of loss to follow-up.
The rinsing of spheres required to eliminate the glucose within the buffer between runs may have resulted in a different subset of spheres being interrogated each time that ultimately could add noise to the system.
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Achieving continuous operation of machine tools in automotive and other manufacturing spheres requires support from condition monitoring professionals.
But finding and studying large numbers of terrestrial-sized spheres requires a major leap in technology (Science, 2 January, p. 30).
Disadvantages of the Bonner system are the weight associated with each sphere and the need to sequentially irradiate the spheres, requiring long exposure periods.
The detailed explanation on how the dielectrophoretic force on the particles stimulates the orderly deposition of the spheres requires further work as particle alignment and enhancement of net electrostatic adhesion force have been described for xerographic applications [31].
Localisation and navigation of the robots are usually performed with optical or magnetic localisation spheres, requiring a pre-procedure import and processing of the 3D data to the robot's workstation, which can be a complex and time-consuming procedure.
In addition, colon cancer spheres require ultra-low-attachment conditions, epidermal growth factor and fibroblast growth factor-2, plus culture for 4 weeks, whereas colospheres form in 1 day, without adding exogenous growth factors.
Thus, less spheres require less packing, or a smaller radius.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com