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However, the multi-layer composition of the polymeric spheres observed in the study is, to our best knowledge, the first report of such structural pattern.
Figure 2 Histogram reflecting the distribution of spheres observed by microscopy in normal liver parenchyma of Pt 2. Six biopsies from Pt 2, with a total volume of 36 mm3, divided into 60 slices were investigated.
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The observation scores recorded by the three human observers were aggregated to generate a detectability score of "sphere observed," "sphere not observed," or "sphere neither observed nor not observed".
The smallest sphere observed was 30 μm, and the largest was 475 μm. Figure 1D shows that the spheres can be mechanically dissociated and can reform spheres upon culturing in USSC growth mediumACF.
Several self-similar generations of spheres in spheres were observed using scanning electron microscopy and transmission electron microscopy.
An enhanced angiogenic effect was also seen in vivo using the chick embryo chorioallantoic membrane assay, where a significant increase in the number of blood vessels converging towards collagen spheres was observed compared with control PCL spheres.
In sphere formation assay, there was almost no qualified sphere in the CD44dim cells, whereas more spheres were observed in the CD44bright cells.
Samples able to form spheres are indicated with open circles and those were no spheres were observed after 30 days in culture with filled circles.
With poly vinyl pyrrolidone) (PVP) emulsifier, PMMA hollow spheres were observed; while with SDS emulsifier, the particles changed from bowl-like particles to hemispheres and truncated spheres with the increase of SDS content.
Three different kinds of morphologies including coexistence of a large number of branched cylinders and a small number of spheres, coexistence of cylinder region and near homogenous region, and coexistence of non-branched cylinders and spheres were observed corresponding to 20, 80 110, and 130 140°C temperature zones, respectively.
The destruction of hollow spheres was observed during viscosity measurement.
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