Sentence examples similar to spheres number from inspiring English sources
Sphere number was determined manually.
Percentage of control sphere number was calculated as (mean sphere number for treated wells/mean sphere number of 0.05% DMSO-treated wells)*100.
Yet the inhibition of ATG4 A led to a decrease in sphere number and size.
Furthermore, the number of spheres was more significantly lower in the secondary spheres than in the primary spheres, and ACCS-M shBra significantly reduced sphere number in comparison to ACCS-M shSOX2.
Cell numbers were counted, and sphere-forming rate at each time point was calculated as the sphere number of 6 days later divided by cell number of the current time point.
Spheres were allowed to form for 14 days at 37 °C and then sphere number was quantified for each well and plotted against the number of cells seeded per well.
After 7 days of incubation, the number of mammospheres was counted using bright-field optical microscopy under a 20× objective lens, and data were presented as the sphere number per 1,000 cells.
Consistent with the in vivo limiting-dilution analyses, a subpopulation of NOTCH1 transformed mammary tumor cells grow in an in vitro tumorsphere assay and importantly, doxycycline treatment significantly reduces sphere number and size.
Also, sphere formation in semisolid culture indicated that both PDGF-AA and bpV pic) could increase total sphere number formed from 10 cells (Fig. 4H-c), and bpV pic), alone or with PDGF-AA, can largely increase the number of spheres with diameters longer than 50 μm (Fig. 4H-b).
It should be noted that the overall number of MCF7 cells that can form spheres in a single cell assay is significantly higher than that in a standard assay, perhaps because cell aggregation leads to an underestimate of sphere number in the standard sphere assay.
