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Although cell-forming self-renewing spheres isolated in this study did not share a unique molecular signature, they all presented a molecular profile highly similar to that expected for neural progenitors and/or neural stem cells [40].
CD44-positive spheres isolated from ovarian serous adenocarcinomas by this technique have been shown to form tumors more efficiently in animal models [ 13].
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Finally, the colloidal carbon spheres were isolated for further characterizations.
After 7 days, spheres were isolated and expression of proteins involved in breast stem cell survival were examined (Figure 3E).
Briefly, total RNA from whole dishes or floating spheres was isolated with RNeasy Micro kits (QIAGEN) according to manufacturer's directions using on-column DNase I digestion and gDNA Eliminator Spin columns (QIAGEN).
Lung tumor spheres were isolated through culture at low density in serum-free culture condition as previously described, which allowed the selection of undifferentiated cancer stem and progenitor cells, while serum-dependent differentiated tumor cells and non-transformed accessory cells were negatively selected [8], [11], [24].
DU145 PC stem-like cells (spheres) were isolated and propagated as we have previously published (Rybak et al, 2011).
The spheres were isolated and implanted subcutaneously in NU/J mice and tumor growth was compared to non-cocultured tumor cells.
The general principles of the model are described and it is tested on three different Stokes flow problems; a single isolated sphere, a pair of otherwise isolated spheres, and a single sphere in a channel.
For cardiomyocyte sphere formation, isolated mouse cells were applied at 5×105cells/cm2 onto glass chamber slides (4 wells; Nalge Nunc International, Tokyo, Japan) and freshly isolated Sk-34 cells were added at 1.5×105 cells/well.
Furthermore, Sox2 staining was detected in the majority of tumor sphere cells isolated from fresh cervical cancer tissues but not from the differentiated cells.
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