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We quantified WT and MU transcript level of expression in these 3 different conditions and confirmed that spheres formation from FD hOE-MSC induces IKBKAP mRNA splicing correction, using RT-qPCR (Figure 8H).
For comparing spheres formation efficiency of colonies, GSCs were transduced with lentiviral particles as described above.
For spheres formation assay, hepatosphere medium was prepared as previously reported [ 34].
But when A2780 sphere cells were cultured with fenretinide in serum-free media, the fenretinide treatment group detected few numbers of spheres formation).
Similar(56)
Sphere formation was readily assessable within several days.
However, while primary sphere formation was reproducibly increased in a dose dependent manner in established cell lines, secondary sphere formation was not increased and neither sphere formation nor CD44+CD24− cells were increased in MDA-IBC-3 tumors cultured with MSC.
Approximately 5% 10% of bulk MCF7S cells were capable of sphere formation (Figure 6B).
Therefore, the two classes of antiestrogens, 4-OHT and ICI, displayed different effects on sphere formation.
In those cases, sphere formation was likely to result from cell aggregation.
The ERα knockdown clones were further characterized for proliferation and sphere formation frequency under antiestrogen treatment.
This suggests that initial cell cell contact immediately after isolation is necessary for sphere formation.
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