BEHIND THE NEWS The experiment involved putting two gyroscopes made of superconducting spheres of niobium into orbit to detect a slight sag and an even slighter twist in space, caused by Earth's gravity and rotation.
Small 1-cm spheres were difficult to detect on the PET images, while clearly visible on TOF-PET reconstructions.
DOI: http://dx.doi.org/10.7554/eLife.00940.017 Although ELISA studies readily detected proinsulin production by IPCs in our 4V spheres, we failed to detect processed C-peptide by ELISA or by immunostaining with antibodies recognizing cleaved C-peptide (data not shown).
The 10-mm sphere was not possible to detect in SPECT images.
This scaling is important when visualizing the smallest lesion in imaging conditions in which the lesions are almost not detectable and allowing one to compare these hard to detect spheres across imaging conditions and systems.
At sphere stage we were able to detect an increase in expression of foxa, klf3, nhsl1b and notum1a in ndr1 mRNA-injected embryos compared to control embryos (see Additional file 2: Figure S1), even though in situ hybridisation is a less sensitive technique than microarray.
It was not possible to detect the 10-mm sphere in any of the SPECT images.
To detect differentiation, U251 tumor spheres were dissociated and cultured in DMEM medium with 10% FBS on coverslips for 48 h.
To examine whether GBM sphere cells have cancer stem cell properties, TaqMan real-time PCR was used to detect the gene expression of glioblastoma stem cell (GSC) markers in GBM sphere cells.
This novel sensor could also be used to detect other heavy metal ions when TiO2 spheres are modified by the relative FH.
