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The embryo, a hollow sphere of cells a few days old, is destroyed in the process.
Blastula, hollow sphere of cells, or blastomeres, produced during the development of an embryo by repeated cleavage of a fertilized egg.
The two known sources of embryonic cells are the blastocyst, a hollow sphere of cells produced a few days after an egg is fertilized, and fetal tissues that hold the future germ cells of the ovary and testis.
The embryo at this stage has no fetuslike features; it is a microscopic sphere of cells that holds an inner clump of cells destined to form all the tissues of the embryo.
Embryonic stem cells, taken from an embryo a few days old at the stage when it is a barely visible, hollow sphere of cells, are expected to play a central role in regenerative medicine since these powerful cells give rise to all the tissues of the adult body.
Moreover, higher grade glioma or oral squamous cell carcinoma cells can form a sphere of cells with higher CSC marker expression compared to lower grade cancers [ 24, 25].
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The spheres of cells were transferred to a Petri dish and cultured in Neurobasal medium (Invitrogen, Milan, Italy) supplemented with B27 (Invitrogen), 20 ng/ml bFGF, and 20 ng/ml EGF (Sigma, Milan, Italy) for 4 7 days.
Somites are generated as epithelial spheres of cells that sequentially bud off at regular intervals from the anterior extremity of the presomitic mesoderm (PSM) in an anterior-to-posterior direction with a strict periodicity, which is controlled by the segmentation clock [2].
Stem cells and cancer stem cells, when studied in vitro, often form free-floating spheres of cells for reasons not completely understood.
In normal brain tissue and in glioblastoma, stem cells were first identified by their ability to form spheres of cells in vitro [ 4, 5].
Poorly differentiated HCC derived SK and undifferentiated HCC derived HLE cell lines efficiently formed spheres of cells (SK-sphere and HLE-sphere), but well-differentiated HCC-derived HuH-7 and Hep 3B cells did not.
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