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The observation scores recorded by the three human observers were aggregated to generate a detectability score of "sphere observed," "sphere not observed," or "sphere neither observed nor not observed".
The smallest sphere observed was 30 μm, and the largest was 475 μm. Figure 1D shows that the spheres can be mechanically dissociated and can reform spheres upon culturing in USSC growth mediumACF.
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However, the multi-layer composition of the polymeric spheres observed in the study is, to our best knowledge, the first report of such structural pattern.
Figure 2 Histogram reflecting the distribution of spheres observed by microscopy in normal liver parenchyma of Pt 2. Six biopsies from Pt 2, with a total volume of 36 mm3, divided into 60 slices were investigated.
The general morphology of the as-prepared ZnFe2O4 hollow sphere products observed by field emission SEM (FE-SEM) and typical images at different magnifications are shown in Fig. 2a c, it was observed that the major morphological feature is a regular spherical shape with an average particle diameter of about 100 210 nm.
Several self-similar generations of spheres in spheres were observed using scanning electron microscopy and transmission electron microscopy.
A rounded bacteriocyte sphere was observed to be transferred into the oocyte at the terminal of ovariole in oocyte III and embedded in mature oocyte.
The diameters of each sphere were observed and measured using an inverted phase microscope (CK-2, Olympus, Tokyo, Japan) fitted with an ocular eyepiece after 5 days.
The more subtle changes of the absorption features of the octahedral iron nitrogen coordination sphere are observed in the FIR spectra (for a temperature-dependent representation of the FIR spectra see Figure S2 in the Supporting Information).
In sphere formation assay, there was almost no qualified sphere in the CD44dim cells, whereas more spheres were observed in the CD44bright cells.
An enhanced angiogenic effect was also seen in vivo using the chick embryo chorioallantoic membrane assay, where a significant increase in the number of blood vessels converging towards collagen spheres was observed compared with control PCL spheres.
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