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At least 100 spermatozoa were evaluated and the sperm vitality recorded as Live to Dead sperm ratio [20].
ALBPS proved to obtain better performance than ALBP when assessing boar sperm vitality, plus it allowed to use a support vector machine (SVM) classifier, improving computational time with respect to the 1×1 matching algorithm proposed in ALBP.
This study was designed to assess the effects of exposing boars to an artificial photoperiod on semen quality in terms of sperm concentration, sperm vitality, sperm motility and acrosome integrity.
In a pilot study, human spermatozoa were found to respond to RF-EMR (at 1.8 GHz with a SAR of 27.5 W/kg) with a range of negative changes including dramatic declines in both sperm vitality and motility.
Sperm viability and acrosomal integrity were assessed using the LIVE/DEAD sperm vitality kit and the acrosomal content marker PSA-FITC, respectively.
In order to evaluate membrane integrity, spermatozoa were incubated with the LIVE/DEAD Sperm Vitality kit (Molecular Probes) as previously described (Amaral and Ramalho-Santos, 2010).
Similar(47)
To investigate the effects of repeated freezing and thawing on human sperm motility, vitality, and DNA integrity.
The particular significance of the present study is that it not only demonstrates a direct effect of RF-EMR on sperm motility, vitality and DNA integrity but also identifies a potential causative mechanism involving electron leakage from the mitochondrial electron transport chain and the induction of oxidative DNA damage.
Variables such as ejaculate volume, sperm motility, vitality, and functional integrity of the membrane did not present significant differences between the three treatments (P > 0.05).
Means were calculated for the separate groups for sample volume, pH, sperm concentration, vitality, rapid progressive motility, total progressive motility, normal morphology and leukocyte count for semen samples.
Because sperm concentration, vitality, rapid progressive motility, total progressive motility, normal morphology and leukocyte count were not normally distributed and nonparametric techniques (Wilcoxon tests) were used to test the significance of the differences between groups regarding these factors.
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