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For sperm morphology evaluation, the strict criteria were used.
Aliquots of the cauda epididymal contents taken postmortem from 26 bulls were used for sperm morphology evaluation.
For sperm morphology evaluation smears were prepared, Papanicolaou stained, and assessed according to strict criteria as described by WHO (1999).
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Sperm morphology was evaluated microscopically.
Sperm morphology was evaluated according to strict criteria.
To assess sperm morphology, we evaluated 200 sperm using the Tygerberg Strict Criteria (Kruger et al. 1988).
The standard evaluation of sperm morphology is performed with phase and/or light microscopy [ 14].
Two ejaculates, one among the first 5 ejaculates and one among the last 3 ejaculates, were chosen for evaluation of sperm morphology.
Assessing fertilizing potential of an ejaculate generally includes tests of sperm function, as well as evaluation of sperm morphology, motility profiles, concentration, viability, ability to acrosome-react and to penetrate oocytes [ 1].
Also, evaluation of sperm morphology according to the type of defect (i.e., head vs. midpiece vs. tail defects) did not isolate any associations that could potentially be masked by evaluating the whole cell only.
Objective: To develop a training program with intervals of continuous quality control assessments for the evaluation of strict sperm morphology.
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