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Novel methods of sperm assessment that are potentially translatable across species require greater consideration.
Sperm assessment is used in the porcine industry for artificial insemination, so the quality of the semen must be higher as possible.
However, in a male rat fertility study with JNJ-26489112, a CNS-active agent, while there were no effects on histological endpoints, mating performance or pregnancy outcomes, sperm assessment was included.
In view of the differences in fertility between rats and humans, these types of sperm effects in rats suggest the potential for an impact on human male fertility that would be undetected if not for the sperm assessment.
There was a significant boar effect on sperm assessment values of PI, H258 and H342 - stained spermatozoa (P < 0.05) and for CASA results (P < 0.05).
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The median interindividual CV was 8.1% for sperm concentration assessment and 11.1% for motility (grade A+B) assessment (Toft et al. 2005b).
To investigate current practices for sperm morphology assessment.
To characterize methodological changes in sperm morphology assessment and to correlate sperm morphology with clinical outcome.
One hundred and thirty-nine ejaculates from 56 adult rams were collected using an artificial vagina, processed for sperm quality assessment and used to perform 1319 AI.
The study aimed to monitor the reading skills of 53 laboratory technologists who are enrolled in a continuous quality control program for sperm morphology assessment.
The final goal of sperm motility assessment is the evaluation of male fertility, the rigorous standardization and validation of the CASA protocol being a pre-requisite to obtain reliable results.
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