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To increase the speed of our method further we are aware of two approaches.
We compared the speed of our method with that of HAPMIX and LAMP-LD.
The speed of our method of about 1 s per pixel is comparable to that of other mass spectrometry imaging systems.
The speed of our method relies on the fact that suffix trees can be constructed in time that is linear in the number of nucleotides analyzed (Gusfield 1997).
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We demonstrate the speed and accuracy of our method by using simulated data.
Therefore, the computing speed and efficiency of our method are higher than those of the BEM.
Making skillful use of the speed and accuracy of our method, exhaustive search for genes of non-coding RNAs that may form pseudoknots is a worthwhile task as another future work.
The morphology, surface coverage and thickness of the final samples are modulated simply by adjusting the experimental conditions such as concentration, temperature and lifting speed, implying the versatility of our method.
In light of all the convenience and speed of our modern methods of communication, is something lost along the way?
To evaluate the speed of our fixation methods we measured temporal loss of viability following the addition of fixative.
In our experiment, no use of SDS-PAGE and LC separation and no use of chemical reductants contribute to the fast speed of our EC/DESI-MS method.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com