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Frailty was defined as having two or more of the following components: (1) unintentional weight-loss, (2) weakness (lowest 20% in grip-strength), (3) self-reported exhaustion, and (4) slow walking speed (lowest 20% 8-m walk-time in seconds).
We divided the set to three quantiles according to translation speed: from the 33% of genes having the fastest translation speed (lowest ribosome density) to those having the slowest speed (high density) in these codon ranges.
This included unintentional weight loss (assessed as ≥5% decrease in self-reported weight that was reported to be unintentional); exhaustion (if response to the question 'Do you feel full of energy?' was 'no'); weakness (assessed as lowest fifth of grip strength distribution); and slow walking speed (lowest fifth of walking speed).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com