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Both the speed and yield of the available transient systems and the natural posttranslational modifications favor these systems above expression in lower eukaryotes, prokaryotes or stable cell lines.
Drastic increases in production speed and yield of recombinant proteins have placed plants in a favourable position [31,32].
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These advantages include vast improvements in surface to volume ratio, energy efficiency, reaction speed and yield and increased control of reaction conditions, to name a few examples.
Due to advantages in terms of speed and yield, attention has recently turned towards the use of transient expression systems, including viral vectors, to produce proteins of pharmaceutical interest in plants.
12 GluC selectively cleaves peptide bonds C-terminal to Glu, but in our studies it was poor in terms of speed and yield.
What matters most is column performance in terms of: sample loading per injection, speed of separation, purity of target and yield of target.
Our study showed that impeller speed (i.e. stirring power input) affects the hydrolysis rate and yield of that particular pretreated spruce to a surprisingly large extent (about 100% yield increase going from 75too 500 rpm) [ 12].
Several studies have shown that cellulase enzymes supplementation can improve the enzymatic hydrolysis of lignocellulosic biomass, in terms of speed and hydrolysis yield.
Experimental designs were employed to study the influence of sodium hydroxide concentration, temperature, liquid and solid flow rates and screw rotation speed or the yield of extracted hemicelluloses.
When the methyl carbonochloridate was reacted with potassium thiocyanate, the potassium thiocyanate was excess, while the drop speed must be slow, as fast speed decreased the yield of product.
Both the fermentation speed and ethanol yield were improved.
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