Moreover, we wanted to evaluate the entire spectrum of stains available in our laboratory that were commonly used in the diagnosis of microbial keratitis.
The spectrum of DHRS7 staining intensity ranges from absent/low (0) to very high/strong (3), from left to right side, respectively, in tested samples.
Nevertheless, DPD expression scores and MLPA data did not reveal a statistical correlation (P=0.461) as tumour specimens without any DPYD rearrangements showed a broad spectrum of DPD staining as well ranging from undetectable up to high expression levels (Table 2).
Unlike that of hnRNP A1, the immunostaining pattern of hnRNP A2/B1 was not as distinct, mainly due to cells in the non-tumour site of the biopsy having a broad spectrum of nuclear staining (several with a score of 2 and 3) when contrasted with the corresponding low levels of hnRNP A1.
The differentially expressed points marked on 2DE spectrum of a graph stained with coomassie brilliant blue, these on the drawing of partial enlargement between calycosin treated and control.
The excitation spectrum of the cells stained with anti-HS-Alexa Fluor 546 and trastuzumab-Alexa Fluor 594 alone was obtained by excitation of the wells at 543 nm and the emission fluorescence was detected from 550 nm to 680 nm, in intervals of 10 nm wavelengths steps, in the fluorometer (FlexStation3, Molecular Devices, Silicon Valley, CA).
e Photograph of stained tissue.
Image analysis software was run to automatically outline and to calculate the sum of green spectrum stained fibrotic area over red background.
The genus Mycobacterium covers a broad spectrum of acid-fast staining species, ranging from harmless saprophytes to significant pathogens [ 1].
Based on the spectrum of percent immunopositive cells, staining categories were determined as follows: (0) No immunoreactivity; (1) <1% of cells immunoreactive; (2)>1–⩽10% immunoreactive; (3) >10 ⩽30% immunoreactive, (4>3030% immunoreactive.
