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The background spectrum of buffer without protein was subtracted from the protein spectra.
The emission spectrum of buffer in the presence and absence of cofactor, which was used as a control, was subtracted from the protein emission spectra.
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Background spectra of buffer samples were subtracted from the respective protein spectra.
The baseline spectra of buffer without protein were subtracted from respective protein spectra.
Spectra of buffer only or buffer with Ca2+ ions were used to correct for light scattering.
The infrared spectra of buffer and residual water vapour were subtracted from the infrared spectrum of the sample.
The ellipticity was monitored from 185 to 250 nm, and the spectra of buffer subtracted postrun.
All spectra were the average of 6 scans with a scan rate of 20 or 50 nm/min and were corrected by subtracting the spectra of buffers.
A CD spectrum of the buffer was recorded and subtracted from the spectrum obtained for the RNA-containing solution.
The background fluorescence spectrum of the buffer was subtracted.
For all the measurements, the spectrum of the buffer was deduced from the sample.
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