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To monitor angiotensin metabolism, the areas of the peaks for different angiotensin forms (Ang II, IV and 1 7) present in the same spectrum, masses 1046.19, 774.92 and 899.02 Da, respectively, were obtained and their summation was arbitrarily assigned as 100%, making it possible to observe the relative changes of each form as a function of time.
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The following molecular weight were identified based on the UV spectrum, mass spectra and fragmentation patterns.
The spectrum mass-tocharge (m/z) were usually acquired with laser shots at 200/spectrum and ranged from 1000 to 4000 m/z.
Leco's ChromaTOF software was used for baseline correction, peak detection, mass spectrum deconvolution, mass spectra library search for identification and calculation of peak height/area.
To support conclusions from FTIR spectra, mass change was investigated with temperature by TGA (Figure 3b).
The acquisition of mass spectra (mass range: 50 800 Da; 1 spectrum/s; resolution: approx. 7500 FWHM) was started immediately.
For the analysis of tandem mass spectra, Mass Frontier™ version 7.0 (Thermo Fisher Scientific GmbH, Bremen, Germany) was used [ 67].
Mass spectra (neutral mass) were deconvoluted using the MaxEnt1 software.
The triple play mode includes a) primary mass spectrum; b) zoom scan mass spectrum; c) MS/MS mass spectrum and d) protein identification from MS/MS).
It produces spectra of masses of molecules from the protein.
Mass spectra and tandem mass spectra were recorded in positive-ion and "high-sensitivity" mode (resolution ~35,000).
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