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Rates were normalised to the tumour volume included within the spectroscopy acquisition slice.
Sagittal, coronal, and axial slices covering the whole liver were preliminarily taken to position the spectroscopy acquisition voxel.
Each of the 46 samples available was a short-echo time (30 ms) single voxel spectroscopy acquisition acquired on a Siemens Symphony 1.5T scanner.
Variability in participant selection, the criteria for conversion to dementia, and methodological inconsistencies in the brain region studied, the spectroscopy acquisition protocol, and the spectroscopy analysis procedures limits the generalizability of the current studies.
By reducing the size of the peptide to a region of MBP that was physiologically relevant to the interaction, we were successful in eliminating aggregation, enabling us to use solution NMR spectroscopy (acquisition parameters shown in Supplementary Table S2).
Single voxel MRS was acquired with the proton brain examination (PROBE) software (General Electric Medical Systems) using a point-resolved spectroscopy acquisition mode (PRESS) sequence (TE = 35 msec, TR = 2 sec with 64 averages) and with automatic shimming to achieve water line widths of < 5 Hz.
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Liver fat content was measured by proton magnetic resonance spectroscopy (H MRS) performed on a 1.5T MR scanner (Intera; Philips Medical Systems, Best, the Netherlands) equipped for proton spectroscopy acquisitions in the afternoon 8 h after a light morning breakfast.
The combined Spectral/FMT system for fluorescence and spectroscopy acquisitions is shown in Fig. 1 and is based on incorporating a spectroscopic module to our existing FMT imager (see Refs. [ 16, 26] for details on the setup).
For MRI and magnetic resonance spectroscopy (MRS) acquisition, anesthesia was induced by inhalation of a mixture of oxygen and 5% isoflurane and maintained with a mixture of oxygen containing 2%2.5%% isoflurane.
The combination of thalamic proton magnetic resonance spectroscopy (10 min acquisition duration) and brain diffusion-weighted imaging (2 min acquisition duration) may increase the diagnostic accuracy of the magnetic resonance scan.
While VP spectroscopy enabled the acquisition of absorbance data for a broad concentration range, PLS modelling allowed for the differentiation between the protein species.
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