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The temperature was then lowered at 1 °C/s to the next temperature/pressure condition where the same spectroscopic acquisition protocol was used.
H NMR spectroscopic acquisition parameters included 32k time domain data points, 512 averages, repetition delay of 5 s and acquisition time of 2.2 s, resulting in a total acquisition time of 1 h 2 min for each spectrum.
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Experimentally accessible ranges of temperature, pressure, magnetic and electric fields available for spectroscopic acquisitions correspond to 3 - 350 K (higher temperatures with additional heating possible), 0 - 60 GPa, 0 - ±9 Tesla and 0 - ±10 MV/m, respectively.
One of the most important such factors is the difficulty associated with achieving adequate shimming over such a large volume of tissue, to the extent required for robust simultaneous spectroscopic acquisitions from each voxel within the matrix [ 14].
The voxel of spectroscopic data acquisition was displayed on consecutive T2-weighted sagittal slices.
JS acquired frozen lymph nodes direct from surgery at Gloucestershire Royal Hospital and was involved in initial spectroscopic data acquisition.
This tissue section was cut adjacent to the section used for spectroscopic data acquisition, and provides a means to directly compare images constructed from HCA analysis with conventional histology.
It is also important to remember that adequate acquisition of spectroscopic data is dependent on the expertise available.
The acquisition of spectroscopic images of this magnitude was quite time-consuming, and could require several hours for very large tissue sections.
The data acquisition, including Raman spectroscopic module (Solis, Andor Technology Ltd ., as well as software control of PPMS temperature and magnetic fields, was implemented using the QD PPMS third party option.
An important part of this scenario is the acquisition of molecular spectroscopic data signatures from key biomolecules produced as a consequence of extremophile survival in stressed environments; a range of terrestrial Mars analogues have been examined including endoliths, cyanobacteria from meteorite impact craters, stromatolites or volcanic and saline rocks [16 21].
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