"Western blot and SILAC mass spectrometry measurements" "In addition to determining protein and mRNA abundances, Schwanhausser et al. (2011) directly measured mRNA and protein degradation rates and calculated the percentage that each contributed to the variance in protein abundances.
Additional factors associated with the H3 K36me3) mark were identified by MudPIT (multidimensional protein identification technology) mass spectrometry, which, in addition to Rpd3 subunits, included several chromatin remodelers (ISW1a, ISW1b, ISW2, and Chd1) and histone chaperones (Spt16, Pob3, Spt6, Rtt106, Hir1, Hir2, and Hir3).
Electron capture dissociation (ECD) and infrared multiphoton dissociation (IRMPD) present complementary techniques for the fragmentation of peptides and proteins in Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) in addition to the commonly used collisionally activated dissociation (CAD).
1) The referees suggest that the authors consider using mass spectrometry to measure 5hmC in addition to immune dot-blot.
Specific points to address are: 1) The referees suggest that the authors consider using mass spectrometry to measure 5hmC in addition to immune dot-blot.
In addition to mass spectrometry we also confirm the identification of the organism by phenotypic strip test (API Coryne®, bioMerieux, Marcy l'Etoile, France).
In addition to 15N emission spectrometry, a new gas chromatography/combustion interface-isotope ratio mass spectrometry/mass spectrometry analytical system was used.
Convergent-beam electron diffraction and analytical electron microscopy, especially the application of energy-dispersive X-ray and electron energy-loss spectrometry, have provided structural and chemical information in addition to strain contrast from lattice defects.
The MPS-I substrate contains the fluorogenic 7-hydroxycoumarin unit, thus allowing this reagent to be used for fluorometric assays of α- l-iduronidase (the enzyme relevant to MPS-I) in addition to tandem mass spectrometry.
