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The accuracy of the Raman line shifts, calibrated by Neon spectral calibration lamp, was on the order of 0.5 cm–1.
Figure 3 Spectral calibration curve.
Spectral calibration was made using amorphous quartz.
This paper describes an experimental setup for the spectral calibration of bolometric detectors used in radioastronomy.
The line of silicon positioned at 519 cm−1 was used for spectral calibration.
OPUS software allowed for automated cosmic spike removal, peak shape correction, and spectral calibration.
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Reliable radiometric and spectral calibrations and accurate atmospheric correction are mandatory in the interpretation of the surface reflectance.
Figure 3 shows the final extracted basis spectral signatures, after these spectral calibrations, corresponding to the four selected tissue types; epithelial tissue, lamina propria, collagen and lymphatic tissue.
We therefore did not perform spectral calibrations on the basis spectra before unmixing to avoid the introduction of unnecessary systematic effects or rounding errors.
For each spectral region, calibration and validation data sets were obtained using simple randomised sampling.
Spectral images calibration to compensate the undesirable effects due to uncontrolled acquisition conditions is done using a standard white reference and a black reference.
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