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In order to test the predictions of such modelling we analysed the X-ray time resolved spectra searching for possible spectral breaks within the observed XRT energy band, finding seven GRBs showing such a break.
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However, given that the original peptide identification search was conducted at a 5% FDR and given the large number of spectra searched, these identifications are incorrect.
The completely digested extracts were eluted on LC-MS system, and the CID spectra searched against the human reference sequence database.
As such, they are a less than ideal substrate for tandem mass spectra search.
When converting scores into E-values based on pooled results out of 10, 000 spectra searches, one looses spectrum specificity.
Spectra search with Sequest, target-decoy peptide filtering, and linear discriminant analysis (Huttlin et al., 2010 ) was performed on raw data obtained from technical duplicate runs on either an Thermo LTQ or a LTQ Orbitrap Elite mass spectrometer.
The current protein sequence databases used by tandem mass spectra search engines, for example IPI, UniProt, and NCBI nr, are designed to be useful as possible to as many researchers as possible.
The contents of constructing the spatial spectra and searching the peak are omitted here.
As shown in Figure S5, the same statistical robustness holds for both centroid and profile spectra while searching the biological protein database tested.
The Thermo Scientific Xcalibur software was used for exporting the spectra and searching the NIST 11 mass spectral database.
Mass spectra and tandem mass spectra were searched against all annotated proteins from strain OM5.
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