Another bellwether is David Hockney's fashionable speculation that the Old Masters secretly relied on optical devices; whatever the merits of this hypothesis, it reflects an increasing readiness to reconsider painting within a spectrum of overlapping visual crafts.
Germline mutations in the RAS-MAPK signalling cascade are found in a spectrum of overlapping developmental syndromes, collectively referred to as the 'RASopathies' (Tidyman and Rauen, 2009).
The spectra consisted of overlapping bands between 1000 and 2000 cm−1 dominated by the "G" band, near ≈1580 cm−1, and the "D" band in the upper 1300 cm−1 range.
Whereas the complementary UV/Vis spectrum showed a broad absorption band because of overlapping spectra of several species including nonbound heme or heme-associated with the protein surface without being distorted, the CD spectra provided additional insight specific to heme molecules internally bound and distorted by the coordination with protein chiral binding sites.
Spectral and temporal filtering is not suitable for this because of overlapping spectra due to simultaneous movements of the foot in the horizontal and vertical planes.
Nevertheless, increasing numbers of overlapping spectra also resulted in the the same trend of better sequencing accuracy.
The H NMR approach does not encounter the issue of overlapping spectra, for it can discriminate the Mb and Hb signals of the Val E11 and the proximal histidyl NδH (Kreutzer and Jue 1991; Kreutzer et al. 1992 , 1993.
This splits the spectra so that adjacent toroidal mode numbers are assigned to different spectra, and the correct amplitudes of overlapping harmonics can be determined.
When applied to sets of overlapping MS spectra, the approach described here also resulted in sequencing accuracy improvements and practically erased the distinction between de novo sequencing of short and long peptides.
Multichannel imaging suffers from an inherent problem of overlapping emission spectra (when the fluorescent markers are simultaneously excited (e.g., [ 34])), where light from more than one fluorescent dye is collected by a given acquisition channel.
