No information that links individuals with serum specimens is available.
Data reported in these studies are compiled from results obtained with serum specimens tested over several years.
All collection and handling equipment in contact with serum specimens were tested for possible OCP contamination.
Results showed that, with serum specimens, the LFA was 100% sensitive when compared with the gold standard blood culture.
Within Group II and III, MUC5AC expression in the pathology specimens did not correlate with serum MUC5AC antibody levels.
Other treatments, including passive immunization with serum specimens from convalescent-phase patients and type 1 interferons, were used to treat SARS patients, with anecdotal success.
The digested specimens were washed with serum-containing medium to inhibit collagenase activity and then placed in 35 mm dishes in Dulbecco's Modified Eagle Medium and Ham's F-12 medium (DMEM/F12, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco-BRL).
In brief, surgically removed fresh specimens were rinsed with serum-free RPMI-1640 (Invitrogen Corporation, USA) for 3 times, cut into 1 mm3 tissue masses and incubated in enzymatic dissociation in DMEM containing 1 mg/mL collagenase IV (Invitrogen Corporation, USA) for 4 6 h at 37°C.
Serum specimens with no reactivity in the first dilution (<8; considered negative) were assigned a titer of 4; serum specimens that showed titers >1,024 were assigned a numerical value of 1,024 for statistical analysis.
If possible, CDC recommends pretreatment of serum specimens with heat (56°C) combined with polyethylene glycol p-tert-octylphenyl ether (Triton X-100) at a concentration of 10 μL/mL of serum to reduce viral titer; however, 100% inactivation may not occur (21 ).
