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The number of specimens with sequence data (ntax), number of variable (# Var), parsimony informative (# Pi), percent parsimony informative (% Pi) and total number of base pairs (bp) is reported.
All specimens with sequence data for ITS2 and for at least one of the two chloroplast markers (rbcL, matK) were included in three datasets (rbcL & matK; rbcL & ITS2; rbcL, matK & ITS2).
Twenty nine specimens failed to provide a sequence for both plastid markers, creating 871 specimens with sequence records for both ITS2 and one of these genes (rbcL & matK from 68%, rbcL & ITS2 from 82%).
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All specimens with sequences consistent with M. tuberculosis were negative for IS 6110.
Composite specimens with stacking sequence [0/90/45/−45]S were impacted at varied impact energies ranging from 5 J to 70 J.
Specimens with a sequence of 100 nm amorphous Cu54Zr46 and 50 nm Cu layers show a compressive flow stress of 2.57 ± 0.21 GPa, matching the strength of pure CuZr metallic glass, hence exceeding the linear rule of mixtures.
We found alterations of the epigenetic machinery in all 4 ATC specimens with genome sequence data.
Analytical tools on BOLD were used to examine patterns of genetic divergence among the 2704 specimens with a sequence ≥500 bp.
The completeness of sampling was visually assessed using the accumulation curve function on BOLD [ 24] for the 2704 specimens with a sequence ≥500 bp, considering both species and barcode clusters (Barcode Index Numbers – BINs [ 81]).
Among 265 (65.6 specimens with successful sequences, 60 (22.6%) were considered uncharacterized; sequence comparison analysis showed high identity to partial HPV nucleotide sequences in GenBank without type designation at this time.
In the three specimens with detectable sequences, we noted that the copy number was very low as nested PCR and replicate testing was often needed for detection.
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