In particular, this experimental study comprises more than 100 foamed concrete specimens with a fixed water/cement ratio and dry densities ranging from around 350 up to 850 kg/m3, two cement types, three foaming agents with either protein or synthetic nature, and curing conditions in water at 30 °C, in air as well as within a cellophane sheet at environmental temperature.
A material testing system (MTS 458.2 MicroConsole™, Minneapolis, MN, USA) was used to rub the titanium specimens alongside the bone specimen with a fixed displacement rate (0.33 mm/s) over a distance of 30 mm.
Tissue specimens were fixed with formaldehyde, dehydrated with alcohol, diaphanized with xylol, included in paraffin, sectioned in slices of approximately 10 µm, applied on a slide and then colored with hematoxylin-eosin.
The specimens are fixed with formalin with methylene blue after surgery, by which physicians are easy to pick up LNs stained by blue dye.
Pancreas and colon specimens were fixed with 4% paraformaldehyde, incubated with 30% sucrose, embedded in OCT compound (Olympus), and frozen in dry ice.
Biopsy specimens were fixed with formalin and stained with Haematoxylin and eosin, and graded as high-grade dysplasia (HGD), low-grade dysplasia (LGD), indefinite for dysplasia (IND), or negative for dysplasia according to the criteria of the Inflammatory Bowel Disease/Dysplasia morphology study group (Riddell et al, 1983).
The specimens were fixed with 10%% formaldehyde solution and decalcified with 10%% ethylenediaminetetraacetic acid (EDTA) (pH 7.4) following delipidation with ethanol.
All specimens were fixed with 2% glutaraldehyde solution, washed with 0.1 M sodium cacodylate buffer, and post-fixed with 1% osmium tetroxide.
The biopsy specimens were fixed with formalin, embedded in paraffin, and stained with hematoxylin and eosin.
The tissue specimens were fixed with formalin, embedded in paraffin and stained with hematoxylin and eosin (H&E).
