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We analyzed IDH mutations using 20 GCTB specimens with direct DNA sequencing (Table 1).
For this study, we analyzed IDH1 and IDH2 mutations using 12 osteosarcoma specimens with direct DNA sequencing (Table 1).
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Steam access and the escape of hydrogen and volatile oxide from void spaces within the brush are restricted compared with specimens with more direct diffusion pathways to the test environment.
Of the 217 specimens with interpretable direct NRA or MODS results, 210 and 207 were interpretable with the LJPM, respectively, and were used in the analysis for sensitivity and specificity.
Pullout specimens were tested with direct tensile loading after being exposed to an alkaline solution (pH 12.9) for 1.5, 3, and 6 months at temperatures of 40 °C, 50 °C, and 60 °C.
The specimens were initially tested with direct immunofluorescence assays (DFAs; Oxoid, Cambridge, UK) for RSV, Flu A/B, PIV1-3, AdV AdV.
This procedure would also make it possible to obtain myocardial specimens from the diseased myocardium, with direct observation of where inflammation is actually occurring.
The method involved dilution (1 1) of the specimens with a 4.0% HNO3, direct injection of a 10 μl aliquot of the corresponding solution into a hot transversely-heated graphite atomizer (110 °C), and application of a fast atomization program (42 s) in which the conventional dry-pyrolysis sequence was substituted by a high-temperature (300 °C) drying step.
For the samples in which there was a single HPV genotype in the specimen, genotyping was readily determined with direct automated DNA sequencing by on-line BLAST sequence alignment algorithms.
Two crucial aspects deserve consideration: (i) many KRAS mutations that occur with low frequencies have not been included in the post- hoc analyses of large phase III randomised trials; (ii) mutation-enriched techniques increase the percentage of KRAS-mutated specimens by about 15%, when compared with direct sequencing (Marchetti and Gasparetti, 2009).
The metallography of 2024 aluminum alloy was studied and compared with the specimens from direct chill casting (DCC) ingots.
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