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In contrast to alternative methods of size adjustment, it has been shown that the geometric mean method effectively equalizes the volumes of specimens while preserving relevant shape information [66].
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Our results show the optical sectioning capability of tissue specimens while mostly preserving image stack photon count, which is usually not achievable with other existing structured light imaging methods.
Using this method, a custom system with shorter CCD-to-specimen distances could achieve higher spatial resolution while preserving displacement measurement accuracy.
Called 3DISCO, the method is a spiritual descendant of Spalteholz's protocol, using a gentler cocktail of chemical solvents that dissolve lipids while preserving cellular structures and dehydrating and hardening the specimen into a clear framework that retains the tissue's original structure2.
We now have numerous specimens of a whole variety of species of feathered dinosaurs, with some species known from hundreds of specimens preserving feathers.
Hard-bodied insects are pinned, while spiders and other soft-bodied specimens are preserved in individual vials stoppered in cotton and then placed in a larger jar filled with ethanol.
The "specimen" category (Ospec) contained: fossil specimens, living specimens, material samples and preserved specimens.
All captured male phlebotomine specimens were preserved in 70% ethanol and taken to the laboratory for species identification, while the females were placed in microtubes containing 6% DMSO and stored at −20°C until being required.
The specimens are beautifully preserved and while it is not exactly clear what killed and buried them, they were apparently able to move at least a little initially before being overwhelmed as they have managed to climb on top of one another.
Typically, plant specimens are preserved by pressing.
All specimens were preserved in 70% ethanol.
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