In this work, high-throughput double cone (DC) specimen, which yielded gradient strain at different regions in a single sample after hot compression, were designed to investigate the thermo-mechanical behaviors of a novel superalloy.
Only T700/M21 specimens were tested in Mode II which yielded an average fracture toughness improvement of 161%.
In 1865, Allen, along with Agassiz, participated in the Thayer Expedition to Brazil, which yielded more than 1400 bird specimens for the collection.
Specificity of the Light Cycler method was determined by testing specimens containing adenovirus, parainfluenza virus and echovirus, all of which yielded negative results with no discernible background.
Potentially causative pathogens were found in all but 1 sample (98%), in contrast to the use of culture alone, which yielded an organism in 38% of specimens.
This is surprising since the procedures used to cultivate M. ulcerans in primary culture were identical to those used for thousands of specimens from patients from other parts of the world (which yielded 45% of positive primary cultures) (11 ).
To minimize noise effects, a long integration time of 4 s was chosen, which yielded a scan time of 36 h for the specimen.
Micrographs were digitized with a Nikon Coolscan 9000ED scanner at a step size of 6.35 μm/pixel, which yielded a pixel size of 1.05 Å/pixel on the specimen.
To identify specimens with gene loss (deletion), a ratio of 0.8 was used as a cut-off, which yielded two samples in this category, both of which were colon tumors (2/81).
As a positive control specimens were stained with a different primary antibody to Smad4 (clone DCS-46, acris, Bad Nauheim, Germany) which yielded results identical to clone B-8 (data not shown).
