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Much effort was expended and the net result was almost literally just a handful of specimens where we had every bone in the tail.
For each 2×2 m quadrat and tree beat sampling, we collected representative samples in the field, recorded the number of individuals of target taxa, and released live extra specimens where we sampled a large number of individuals, and where species were readily recognisable.
We did not use bone specimens where we could macroscopically see both solid bone and bone marrow, so we did not use any additional washing steps to remove bone marrow.
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In order to confirm original diagnosis, for specimens where tumor blocks were available, we performed immunohistochemistry for calretinin and WT-1.
The region illustrated is typical of normal epidermis (well separated from the lesion), which we examined in all specimens where it was present, totalling at least several hundred melanocytes.
Moreover, we also have successfully used the device on seven clinical serum specimens where HBV DNA levels have been confirmed by real-time PCR.
We found a demonstrable ALL in ten out of 11 specimens and in all specimens where an intact ACL was found.
We observed significantly stronger CD44 expression in resected endometrioid than in papillary serous cancer specimens, where the molecule was virtually absent.
We saw some evidence of potentially intact loriciferan tissue in some of the recovered specimens, where two Rose Bengal stained specimens each had a possible oocyte (Fig. 5b, Additional file 6: Figure S6).
This could conceivably have resulted in that some positive HPV-tests derive from specimens where cancer tissue was not present.
The splitting tensile deformations are introduced to generate microcracks in ECC specimens, where cylindrical specimens were pre-loaded to different deformation levels.
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