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Specimens were washed with PBS/1%Tween.
After saturation the specimens were washed, dried and weighed.
The specimens were washed in the same buffer before and after fixation.
After 24 h of immersion, the specimens were washed and reweighed.
Steel specimens were washed with distilled water, degreased with acetone and dried in hot air oven for 5 min.
The specimens were washed with soap solution, degreased with acetone, dried in air oven and weighed [13].
Prior to the experiment, specimens were washed with distilled water, degreased in acetone, dried, and stored in a vacuum desiccator.
The specimens were washed in the same buffer before and after post-fixation and then rinsed in distilled water.
Prior to the coating procedure, the specimens were washed, sonicated in dry ethanol for 2 min and dried with cyclohexane and acetone.
The specimens were washed and then stained with Concanavalin A-tetramethylrhodamine conjugate (Invitrogen, Carlsbad, CA, USA) and SYTO® 9 (Invitrogen) for 30 min in the dark.
After staining, the specimens were washed twice with PBS.
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