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The specimens were sorted into 10 groups based on (i) specimen size, (ii) theoretical lateral FRP confining pressure, (iii) number of layers of FRP wrap, and (iv) inclusion and exclusion of internal steel reinforcement.
Within each group, specimens were sorted by descending average NCC (summed over rows/columns) to clarify the pattern.
Specimens were sorted to family, genus, or species when possible and were preserved in 95% ethanol which was changed once after 24 hours.
The specimens were sorted into three sets: Bright (B, 19 specimens), a random sample from the 3% brightest specimens; Dim (D, 24 specimens), a random sample from the 3% dimmest ones; and All (A, 25 specimens), a random sample from the remaining worms equally distributed over the full range of observed brightness values excluding top 3% and bottom 3%.
Specimens were sorted under a dissecting microscope.
Then the specimens were sorted, washed and oven-dried.
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The chromosomes obtained from the cell line of a male specimen (TK165824) were sorted on a MoFlo dual-laser cell sorter and isolated according to size and base-pair composition [ 16] at the Wellcome Trust Sanger Institute, UK.
Notably, specimens with intermediate morphologies were sorted close to the midpoint root of the tree topology.
Sequences were sorted to specimens only if they perfectly matched primer barcode sequence.
All specimens caught in all years were sorted to the family level except for collembolans and mites who were only counted.
Discarded surgical specimens were enzymatically digested and c-kit-positive hCSCs were sorted by FACS.
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