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If no visual bacterial growth was detected during the incubation time, the bone specimens were snap frozen with liquid nitrogen and pulverized with a mortar and a pestle.
Tumor specimens were snap frozen in liquid nitrogen and stored at −80 degrees Celsius.
The specimens were snap frozen in liquid nitrogen and sections were prepared by cryostat sectioning.
The tissue specimens were snap frozen in liquid nitrogen pre-cooled isopentane and stored in liquid nitrogen or at −80°C until further processing.
Biopsy specimens were snap frozen in Optimal Cutting Temperature immediately after endoscopic resection and stored at −80°C for histological immunostaining or homogenized to extract protein for western immunoblotting or RNA for real time PCR.
Briefly, lungs specimens were snap frozen in liquid nitrogen.
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Specimens were snap-frozen immediately after resection.
Tissue specimens were snap-frozen immediately following biopsy or surgical removal and stored in liquid nitrogen until further processing.
For microscopic evaluation, colonic tissue specimens were snap-frozen in liquid nitrogen and stained with hematoxylin-eosin (HE).
Specimens were snap-frozen in liquid nitrogen or deposited in RNALater (Qiagen, Germany) and subsequently stored at −80°C.
All specimens were snap-frozen in liquid nitrogen.
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