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Human specimens were separately analyzed with respect to progenitor phenotypes to reveal individual to individual variability.
The specimens were separately stained with hematoxylin and eosin (H&E), periodic acid Schiff stain, and Alcian blue stain.
Isolated chondrocytes from individual specimens were separately cultured with DMEM/F-12 plus 10% FBS at 37°C under a humidified 5% CO2 atmosphere until reaching confluence.
Isolated chondrocytes from individual specimens were separately cultured with Dulbecco Modified Eagle Medium/Ham F-12 (DMEM/F-12) (GIBCO BRL, Paisley, UK) plus 5% fetal bovine serum (FBS; Invitrogen, Life Technologies, Paisley, UK) at 37οC under a humidified 5% CO2 atmosphere until reaching confluence for 4 to 6 days.
Isolated chondrocytes from individual specimens were separately cultured with DMEM/F-12 (GIBCO, Life Technologies, Paisley, UK) plus 5 % FBS (Invitrogen, Life Technologies, Paisley, UK) at 37 °C under a humidified 5%% CO2 atmosphere until reaching confluence for 4 6 days.
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Each specimen was separately attached to filter paper and fixed immediately.
Seventy-four suresected reSRcted (SR) and 201 ER specimens were analyzed separately.
The specimens were immersed separately in 50 mL of 0.2%% acidulated phosphate fluoride (APF) solution with pH 5.0 at room temperature (25 °C) for various periods.
Specimens were immersed separately in 50 mL of 0.2%% acidulated phosphate fluoride (APF) solution with pH 5.0 at room temperature for 16 h.
Before the construction process of the test specimens at the laboratory shown in Fig. 7, beam and column elements of precast specimens were constructed separately in a precast facility and then transported to the laboratory.
Specimens were immersed separately in 50 mL of 0.2%% acidulated phosphate fluoride (APF; 0.048 M NaF + 0.018 M H3PO4) solution with pH 5.0 at room temperature (25 ± 2 °C) for 16 h.
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