The Illumina reads for each specimen were screened using the FASTX-Toolkit [ 19] to remove the first 15 and last 6 nts.
The breast cancer specimens as well as the autologous blood samples were screened using 6 polymorphic microsatellite markers spanning chromosome band 16q22 containing the ATBF1-A gene.
Colonies were screened using colony PCR.
was screened using the Heme Interaction Assay.
A total of 26,746 swab specimens were screened by using reverse transcription PCR.
The 189 serum specimens were screened by using the Mini-PROTEAN II Multiscreen Apparatus (Bio-Rad, Hercules, CA, USA).
All specimens were screened for rotavirus by using a commercial enzyme-linked immunosorbent assay (ELISA) (Premier Rotaclone, Meridian Diagnostics, Cincinnati, OH, USA) with monoclonal antibodies specific for group A human rotavirus, according to the manufacturer's protocol.
87 patient specimens were screened for anti-HCV, using COBASe411 eCLIA, Roche, Germany), Axsym (MEIA, Abbott, Germany), HCV Qualisa (ELISA, Qualpro, India) and HCV Tridot (Rapid Immunofiltration, J. Mitra, India), and for presence of HCV RNA using COBAS TAQMAN 48 analyzer (Roche).
Inactivated bat serum specimens were screened for NiV antibodies by using a serum neutralization test.
The serum specimens were screened by indirect immunofluorescence assay by using XJ0625-infected XJ0625-infected BHK-21 BHK-21
