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Regardless of source, DNA specimens were quantified using human-specific Alu-PCR to confirm a minimum level of amplifiable human DNA [ 17] and randomized prior to genotyping.
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The immunoreactivity for IL-6 and TGF-β1 in the lung cancer specimens was quantified using previously validated procedures [41], [42]: no staining (grade 0), weak but detectable focal staining (grade 1), moderate staining (grade 2), marked staining (grade 3), very intense staining of the tumour cells (grade 4).
The concentration of the protein extracted from each specimen was quantified using the BCA protein assay kit.
The expression level of 470 mature miRNAs found their biopsy specimen, obtained prior to the combination therapy, were quantified using microarray analysis.
The expression levels of microRNAs were quantified using microarray and RT qPCR in GIST specimens from patients treated with neoadjuvant imatinib.
Methods: The levels of hTERT-mRNA were quantified using real time RT-PCR in lesional versus nonlesional skin specimens from 24 psoriatic patients.
Data were quantified using MetaXpress software.
All images were quantified using ImageJ (NIH).
NRF2 images were quantified using ImageJ.
FRET measurements were quantified using ImageJ (NIH).
The 8-OHdG images were quantified using ImageJ.
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